RasGRP3 is expressed in human breast derived ductal adenocarcinoma. (A) Q-PCR and (B) Western blot analysis of RasGRP3 and phosphoRasGRP3 expressions in human normal breast (Controls; n = 21) and breast derived ductal adenocarcinoma (Tumors; n = 33) with various tumor grades (G1-G3) presented according to the molecular grading of human breast derived ductal adenocarcinoma (Luminal A; Luminal B and HER-2). GAPDH was used as an internal control. The results are representative of three independent experiments for Q-PCR and two independent experiments for Western blot. Values represent the mean ± SEM. (C) Representative images of RasGRP3- and phosphoRasGRP3-specific immunreactivity with diaminobenzidine as a chromogen (brown staining) on human normal and breast derived ductal adenocarcinoma sections. Human kidney samples were included as a positive control (PC) . Nuclei were co-stained by Mayer’s Hematoxylin (blue staining). Preabsorption negative control (NC) was used. Images were taken at magnifications 20x. The results are representative of four independent experiments.