Down-regulation of RasGRP3 expression induces apoptosis in MCF7 derived cells. (A) Representative images of MCF7 and T-47D derived cells stained with Annexin-V and PI and analyzed by flow cytometry. Data were evaluated with FSC Express softwer and presented in the percentage of gated cells. Values represent the mean ± SEM for three independent experiments. (B) Assessment of mitochondrial membrane potentials of MCF7 and T-47D derived cells by fluorimetric DilC1 (5) assay with values normalized to the levels of non-transfected control cells. Values represent the mean ± SEM for four independent experiments.