Suppression of RasGRP3 expression inhibits xenograft tumor growth of both MCF7 and T-47D cells. SCID mice were injected subcutaneously with 4x106 cells of the indicated MCF7 and T-47D derived cells (n = 4 mice for each group). The animals were sacrificed 12 weeks after injection, and the tumors were excised, weighted (A) and processed for immunohystochemical analysis. Values represent the mean ± SEM of four independent experiments. (B) Digitalized images of Ki67-specific immunreactivity (Additional file 3: Figure S1 and Additonal file 4: Figure S2) obtained on sections prepared from tumors developed by MCF7 and T-47D derived cells were analysed using Image J image analysis softwer. In every each section the number of cells were counted at 5 randomly placed, equal areas of interest (AOI) and the average of Ki67 imunnopositive cells of the 5 AOI was defined. Ratios: in T-47D shSCR n = 1213/1098; in T-47D shRasGRP3 n = 1216/1096; in MCF7 shSCR n = 1268/757; in MCF7 shRasGRP3 n = 1112/553. The numbers represent total cell number/Ki67 positive cells ratios. Values represent the mean ± SEM.