Effects of down-regulation of RasGRP3 on the Ras signaling pathway II. IGF1R, EGFR and HER-2 expressions were detected by Q-PCR and immunoblotting of RasGRP3 knockdown cell lines created from MCF7 and T-47D cells. GAPDH (Q-PCR) and β actin (Western Blot) were used as internal controls. The results are representative of three (Q-PCR) or two (Western blot) independent experiments. Values represent the mean ± SEM.