Figure 1

The overexpression of MIR31 enhanced tumorigenesis in vitro and in vivo. (a) Establishment of HEC-50B-expressing MIR31 cells. The results of the qRT-PCR analysis of the expression levels of MIR31 are shown in the bar graph. *p < 0.05, unpaired two-tailed Student’s t-test. The experiments were performed in triplicate. (b) Colony formation assay with 1% FBS. Representative stained colonies are displayed in the left panel. *p < 0.05, unpaired two-tailed Student’s t-test. The experiments were performed in triplicate. (c) Subcutaneous tumors in the seven nude mice are displayed in the left panel. The weights of tumors are shown in the right bar graph. *p < 0.05, paired two-tailed Student’s t-test.