MIR31 regulates the LATS2 expression by inhibiting translation. (a) The potential binding site for MIR31 in 3’UTR of LATS2 mRNA. (b) The expression level of LATS2 in the mock and MIR31-overexpressing cells (top). The LATS2 expression was increased by the MIR31-specific inhibitor (bottom). The results of immunoblotting for LATS2 and α-tubulin are shown. (c) The luciferase activity after transfection of the indicated 3’-UTR-driven reporter constructs. Reporter plasmids containing no oligonucleotides as a Control, the wild-type 3’UTR region of LATS2 as a Wild type and the mutant 3’UTR region as a Mutant. *p < 0.05, unpaired two-tailed Student’s t-test. (d) Colony formation assay (bar graph) and immunoblotting for LATS2 and α-tubulin following shRNA transfection (bottom panel). *p < 0.05, unpaired two-tailed Student’s t-test. All experiments were performed in triplicate.