Correlation between the MIR31 expression and the immunohistochemical detection of LATS2 and CCND1 in human EC cells. (a) Proportion of patients with positive staining for LATS2 and CCND1 classified into the MIR31 > =15 and MIR31 < 15 groups. *p < 0.05, Chi-square test. (b) The MIR31 expression assessed using qRT-PCR with RNU44 as the endogenous control (top) and representative micrographs, x100. (c) The MIR31 expression levels in all patients classified according to staining for LATS2 and CCND1 are shown in the scatter diagram. The horizontal solid lines indicate the mean. (d) The expression levels of MIR31 in the human EC cells were analyzed using qRT-PCR. The horizontal lines demonstrate the mean. *p < 0.05, Mann-Whitney's U-test.