Figure 6

Tumor onset in the intestinal tract of Apc^Min/+/ Vash2^-/- mice. A, Genomic DNA samples isolated from mouse tails were genotyped by PCR using primers that detect both WT and mutant alleles of Apc^Min/+ or Vash2. B, Dissection micrographs of the small intestine from Apc^Min/+ mice (left) and Apc^Min/+/Vash2^-/- mice (right) at 16 weeks of age. C, Polyp numbers in the small intestine in Apc^Min/+ mice (open column) or Apc^Min/+/Vash2^-/- mice (closed column) at 16 weeks of age. ^**P < 0.01, n = 8. D, LacZ activity in Apc^Min/+mice, Apc^Min/+/Vash2^+/- mice and Apc^Min/+/Vash2^-/- mice. Expression of LacZ was not seen in Apc^Min/+ mice or in Apc^Min/+/Vash2^+/- mice. Note the marked LacZ expression in crypts (arrows) and tumor cells in Apc^Min/+/Vash2^-/- (arrowheads). Line, tumor area.