Figure 8

Tomato lectin staining of functional tumor vessels in gastrointestinal polyps from Apc^Min/+/ Vash2^-/- mice. A, Tomato lectin staining of functional tumor vessels in polyps. Double staining for tomato lectin and CD31 staining of tumor vessels in tumor lesions (a). Double staining for tomato lectin and CD31 staining of tumor vessels in hyperplastic lesions (b). Note the tumor lesions in Apc^Min/+/Vash2^-/- mice were less vascularized than hyperplastic lesions in the gastrointestinal tract. Scale bars, 100 μm. B, MVD was estimated by measuring the total area of tomato lectin⁺ blood vessels in 5 separate fields of hyperplastic or tumor lesions in Apc^Min/+/Vash2^-/- mice at a magnification of × 400. ^*P < 0.05, n = 3.