Figure 2
From: Melanoma cells influence the differentiation pattern of human epidermal keratinocytes
Detection of a panel of keratins (green signal, A-L, I 2 -L 2 ), keratin 14 (red signal, A, A 1 , E, E 1 , I, I 1 , I 3 ), keratin 8 (red signal, B, B 1 , F, F 1 , J, J 1 , J 3 ), keratin 19 (red signal C, C 1 , G, G 1 , K, K 1 , K 3 ) and vimentin (red signal, D, D 1 , H, H 1 , L, L 1 , L 3 ) in keratinocytes cultured on 3 T3 feeder cells in presence of BLM melanoma cells (A-D, A 1 - D 1 ), of highly pigmented neonatal melanocytes (HPM, E-H, E 1 -H 1 ) and of neural crest stem cells (NCSC, I-L, I 1–3 -L 1–3 ). The co-localization of signal has been verified by the measurement of fluorescence profile (I3-L3). The figures show that BLM and NCSC have a similar stimulatory effect on the expression of all the studied markers. Scale bar denotes 50 μm.