Figure 2
From: NF-κB induces miR-148a to sustain TGF-β/Smad signaling activation in glioblastoma
miR-148a directly targets QKI. (A) Predicted miR-148a target sequence in QKI-3′UTR and mutant containing three mutated nucleotides in the seed sequence of miR-148a (miR-148a-mut). (B) WB of QKI expression in negative control 1 (NC1)- or miR-148a–transduced cells or cells transfected with NC2 or miR-148a inhibitor. β-Actin was used as the loading control. (C) MiRNP IP revealing the association of miR-148a with QKI. MIG6 and GAPDH were used as positive and negative controls, respectively; 5S rRNA was used as a control for overall expression levels. (D) WB analysis of GFP expression. (E) Luciferase assay of cells transfected with pGL3-QKI-3′UTR reporter with miR-148a mimic (10 or 50 nM), miR-148a mutant, or miR-148a inhibitor (20 or 100 nM).