Figure 6

Effects of co-treatment on non-malignant bronchial epithelial cells. (A) Bronchial epithelial cells (BE) were isolated from explanted healthy human lungs (see Materials and methods for details) and kept in cell culture under normoxic conditions up to 4th passage. H23 and BE cells were treated with panobinostat, cisplatin or combination of both for 24 or 48 hours, followed by cell viability measurement. (B) Upon different treatments of H23 and BE cells for 48 hours the Caspase-3 Intracellular Activity Assay Kit I (PhiPhi-Lux®) was performed, followed by FACS analysis. Data are presented as percentage of cells positive for active caspase-3. (C) Cell morphology of non-malignant BE and malignant H23 cells 48 hours upon different treatments. Scale bar = 100 μm. ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; determined by Student’s t-test. Pano, panobinostat; Cis, cisplatin.