Figure 1

Downregulation of miR-146a in human HCC cell lines and tissues. A. miR-146a expression was examined in paired primary HCC tissues (T) and their match adjacent non-tumor tissues (NT) of 11 individual patients. The miR-146a levels were normalized to RUN6 expression. B. miR-146a is decreased in HCC tissues compared with in matched non-tumor liver tissues (n = 96; NCBI/GEO/GSE 22058). P value was calculated by Student’s test. C. and D. Real-time RT-PCR analysis of miR-146a expression in human normal liver tissues and HCC cell lines (C)., including SMMC-7721, Huh-7 and HepG2, and in 53 HCC cases (D). miR-146a levels were normalized to RUN6 expression. P value was calculated by Student’s test. E. Real-time RT-PCR analysis of miR-146a expression in 28 big HCC tissue samples (≥ 5 cm) and in 15 small HCC tissue samples (< 5 cm) was determined by real-time RT-PCR and normalized to the level of RUN6. P value was calculated by Student’s test. F. Real-time RT-PCR analysis of miR-146a expression in 26 HCC tissue samples with metastasis and in 17 HCC tissue samples without metastasis was determined by real-time RT-PCR and normalized to the level of RUN6. P value was calculated by Student’s test.