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Figure 2 | Molecular Cancer

Figure 2

From: microRNA-146a inhibits cancer metastasis by downregulating VEGF through dual pathways in hepatocellular carcinoma

Figure 2

The association between the expression and promoter methylation of miR-146a in human liver cancer tissues. A. Real-time RT-PCR analysis of miR-146a expression in SMMC-7721 cells treated with 5-aZa-dC for 24 h. Error bars represent ± SD. A student t-test was employed. (*, p < 0.05) (**, p < 0.01). B. Diagram of the locations of the CpG sites. Vertical bars represent CpG sites in the promoter of miR-146a.C. Bisulfite genomic sequencing analyses of the miR-146a CpG site in normal liver tissues (n = 6) and HCC tissues (n = 22). The filled and open boxes are represented methylated and unmethylated CpG, respe60ctively. Ten single clones are represented for each sample. Open boxes, 0% methylation; light filled boxes, 0-50% methylation; black filled boxes, 50-100% methylation. NL, normal liver; HM, HCC with metastasis; HN, HCC without metastasis. D. the association between methylation status of candidate position and HCC metastasis. Error bars represent ± SD.

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