Figure 3
miR-146a inhibited cell invasion and metastasis in vitro and in vivo . A. Changes in miR-146a levels in SMMC-7721 and Huh-7 cells transfected with miR-146a or miRNA control (miR-Ctrl) for 48 h as measured by real-time RT-PCR. the datum represents the mean ± SD. B. Quantification of wound healing assay of SMMC-7721 cells transfected with miR-146a or miRNA control (miR-Ctrl). the datum represents the mean ± SD. *p < 0.05. C. In vitro invasion assay. Cells from the same transfection as in (B). were seeded into triplicate mitrigel coated invasion chambers at 24 h post-transfection and allowed to invade toward serum for 24 h. The invading cell numbers on each filter were counted and data were plotted. the datum represents the mean ± SD. The student’s t test was used to compare the difference between two groups. *p < 0.05. D. Changes in miR-146a levels in Huh-7 and HepG2 cells transfected with antagomiR-146a or nonrelated control moleculars (NC) for 48 h as measured by real-time RT-PCR. the datum represents the mean ± SD. E. Quantification (right) of wound healing assay of Huh-7 and HepG2 cells transfected with antagomiR-146a or nonrelated control moleculars (NC). The student’s t test was used to compare the difference between two groups. the datum represents the mean ± SD. *p < 0.05. F. In vitro invasion assay. Cells from the same transfection as in (E) were seeded into triplicate mitrigel coated invasion chambers at 24 h post-transfection and allowed to invade toward serum for 48 h. The invading cell numbers on each filter were counted and data were plotted. the datum represents the mean ± SD. The student’s t test was used to compare the difference between two groups. *p < 0.05. G. Representative images of the liver, spleen and number of visible liver metastases in vector- or miR-146a treated mice. Nude mice were injected under the spleen capsule with 2 × 106 vector or miR-146a stable transfected SMMC-7721 cells via a 27-gauge needle (n = 10 in each group). H. Tumor nodule number were counted (n = 10 in each group). Data are mean + SD. * p < 0.05.