Figure 6

Correlation between miR-146a and HAb18G/CD147, VEGF, NF-κB p65 expression, and prognosis in HCC tissues. A. Relative expression of HAb18G/CD147 in HCC tissue and in normal liver tissue by real-time RT-PCR. B. Staining score of HAb18G/CD147 in HCC tissue and in normal liver tissue by immunohistochemical staining. C. Relative expression of HAb18G/CD147 in immunohistochemical staining positive and negative HCC tissue by real-time RT-PCR. D. Representative images of immunohistochemical staining for HAb18G, VEGF and NF-κB p65 in low and high miR-146a expression cases (left). Scale bar represents 100 mm. Bar charts show the association between miR-146a expression and HAb18G, VEGF and NF-κB p65. The low and high miR-146a expression was stratified by using average of miR-146a expression as threshold. The y-axes represent HAb18G, VEGF and NF-κB p65 expression, as measured by immunohistochemical staining. Error bars represent ± SD. *p < 0.05, ** < 0.01. E. Kaplan-Meier metastasis-free survival curves in miR-146a low and high expression HCC cases. The low and high miR-146a expression was stratified by using average of miR-146a expression as threshold.