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Figure 1 | Molecular Cancer

Figure 1

From: The anti-angiogenic and cytotoxic effects of the boswellic acid analog BA145 are potentiated by autophagy inhibitors

Figure 1

BA145 exhibits antiproliferative and anti-angiogenic properties. (A) MTT cell viability assay of PC-3 cells and HUVECs treated with different concentrations of BA145 for 24 and 48 h. (B) FACS analysis of SubG1 cell populations following BA145 treatment of PC-3 cells and HUVECs at the indicated concentrations for 24 h. (C) Flow cytometric analysis of MMP loss following BA145 treatment of PC-3 cells and HUVECs at the indicated concentrations for 24 h. (D) Effect of BA145 on VEGF induced angiogenesis in vivo. Six week old C57/BL6J mice were injected with Matrigel containing 50 and 100 mg/kg BA145 along with 100 ng of VEGF into the ventral area. After 10 days, animals were sacrificed to remove Matrigel plugs and were photographed. The neovascularization of the Matrigel plugs were quantified spectrophotometrically using Drabkin’s reagent. (E) VEGF induced chemotactic motility in PC-3 cells and HUVECs following treatment with the indicated concentrations of BA145 for 10 h. Migrated cells were counted manually, and the percent inhibition of cell migration was calculated as shown. Columns, mean; bars, SD; with ***p < 0.001, **p < 0.01, *p < 0.05 versus control.

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