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Figure 3 | Molecular Cancer

Figure 3

From: The anti-angiogenic and cytotoxic effects of the boswellic acid analog BA145 are potentiated by autophagy inhibitors

Figure 3

Effect of autophagic inhibitors on the antiproliferative properties of BA145 in PC-3 cells. (A) PC-3 cells were co-treated with BA145 (30 μM) and/or the autophagy inhibitors ammonium chloride (10 mM), 3-MA (5 mM), or LY294002 (20 μM) for 24 h. Cell viability was assessed by an MTT assay. (B, C) Analysis of SubG1 cell populations and MMP loss in BA145 treated PC-3 cells. Cells were pretreated with autophagy inhibitors for 1 h followed by addition of BA145 (30 μM) for the next 24 h. PI and Rh-123 dyes were used for the analysis of cell cycle and MMP loss, respectively using flow cytometry. (D) Effect of autophagic inhibitors on the cleavage of procaspase-3 and PARP-1 in BA145 (30 μM) treated PC-3 cells after 24 h. (E) Effect of BA145 on procaspase-3 and PARP-1 cleavage in LC3 silenced cells.

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Molecular Cancer

ISSN: 1476-4598