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Figure 5 | Molecular Cancer

Figure 5

From: The anti-angiogenic and cytotoxic effects of the boswellic acid analog BA145 are potentiated by autophagy inhibitors

Figure 5

Autophagy inhibition significantly enhances BA145 mediated anti-angiogenic activity . (A) Aortic segments were isolated from Sprague-Dawley rats and placed in Matrigel-covered wells. BA145 (10 μM) was added to the wells along with ammonium chloride (N; 10 mM), 3-MA (5 mM), or LY294002 (10 μM) for 4 days. Microvessel sprouting was quantitated with Image J software. (B) Effect of autophagy inhibition on BA145 mediated suppression of VEGF-induced tube formation. HUVECs were seeded in 24-well plates coated with Matrigel and co-treated with BA145 (10 μM) and autophagy inhibitors for 8 h. Cells were fixed and tubular structures were photographed. (C) Percentage inhibition of microvessel sprouting and tube formation of endothelial cells by BA145 along with autophagy inhibitors. (D) Colony formation assay of PC-3 cells. Cells were treated with BA145 (30 μM) along with the indicated autophagy inhibitors for 24 h. Cells were trypsinized and 1000 viable cells were seeded in 60 mm dishes. Cells were allowed to form colonies for 15 days, stained with 1% crystal violet, and counted manually. Columns, mean; bars, SD; with ***p < 0.001, **p < 0.01, *p < 0.05 versus BA145 alone.

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