Figure 6

Autophagy inhibition potentiates the anti-angiogenic effects of BA145 in vivo. (A) Effect of BA145 and CQ on VEGF induced angiogenesis. Six weeks old C57BL/6 J mice were injected with Matrigel containing BA145 (50 mg/kg), CQ (50 mg/kg), or both BA145 and CQ along with VEGF (150 ng) into the ventral area (6 mice per group). Matrigel plugs were monitored 10 days later. (B) Matrigel plugs were fixed, sectioned, and stained with H&E. (C) Neovascularization of Matrigel plugs were quantified with Drabkin’s reagent using a spectrophometer. (D) Effect of BA145 and CQ on tumor growth in vivo. PC-3 M-luc2 cells implanted into NOD.SCID mice were treated with vehicle, BA145 (75 mg/kg), BA145 (75 mg/kg) plus CQ (50 mg/kg), positive control flutamide (25 mg/kg), and CQ (50 mg/kg) on alternate days for 28 days. Tumor volumes are reported as mean ± SE. (E) Representative pictures of excised tumors and their mass. (F) Western blot analysis of the indicated proteins in tumor tissues. β actin was used as loading control. Columns, mean; bars, SD; with ***p < 0.001 versus BA145 alone.