Figure 1From: Gene expression profiling by DNA microarray analysis in mouse embryonic fibroblasts transformed by rasV12 mutated protein and the E1A oncogeneA. Expression of RAS was verified by immunoblot analysis in MEFs transduced with pBabe (control) or pBabe-rasV12/E1A (transformed) retroviruses. B. Morphological aspect of the pBabe and pBabe-rasV12/E1A transduced mouse embryonic fibroblats. C. Anchorage-independent growth of the rasV12/E1A transformed MEF. Fifty thousand cells were plated on 0.6% agar in DMEM-10% FCS and overlaid on 0.6% agar in the same medium. Photomicrographs were taken 10 days after plating. D. rasV12/E1A transformed MEF induce tumor formation. One million of pBabe and pBabe-rasV12/E1A transduced mouse embryonic fibroblast were injected in 200 μl PBS as xenografts in nude mice. Representative mice at day 18.Back to article page