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Figure 1 | Molecular Cancer

Figure 1

From: Gene expression profiling by DNA microarray analysis in mouse embryonic fibroblasts transformed by rasV12 mutated protein and the E1A oncogene

Figure 1

A. Expression of RAS was verified by immunoblot analysis in MEFs transduced with pBabe (control) or pBabe-rasV12/E1A (transformed) retroviruses. B. Morphological aspect of the pBabe and pBabe-rasV12/E1A transduced mouse embryonic fibroblats. C. Anchorage-independent growth of the rasV12/E1A transformed MEF. Fifty thousand cells were plated on 0.6% agar in DMEM-10% FCS and overlaid on 0.6% agar in the same medium. Photomicrographs were taken 10 days after plating. D. rasV12/E1A transformed MEF induce tumor formation. One million of pBabe and pBabe-rasV12/E1A transduced mouse embryonic fibroblast were injected in 200 μl PBS as xenografts in nude mice. Representative mice at day 18.

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