Figure 4

RT-PCR amplification of ATM mRNA in the different cells using the primer pairs listed in Table2. Human foreskin fibroblasts, human rhabdomyosarcoma cell lines were cultured and the total RNAs were isolated from the cultured cells. The RT-PCR was performed using the total RNA from the cells, and the PCR products were separated on 1% agarose gel and visualized by staining with ethidium bromide. The lengths of the PCR products were compared with each cell line used.