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Figure 1 | Molecular Cancer

Figure 1

From: Interfering with TGFβ-induced Smad3 nuclear accumulation differentially affects TGFβ-dependent gene expression

Figure 1

Upregulation of TGFβ target genes is diminished by SB-202190 and SB-203580. A and B, Real-time RT-PCR measurement of PTHrP P3 promoter and PAI-1 transcripts. Cells were incubated with 5 ng/ml TGFβ for four hours. C and D, Transient transfection assays using the 3TP-Luc reporter plasmid. Cells were treated with TGFβ for seven hours, lysed and luciferase activity was measured and normalized to β-galactosidase activity. Cells were incubated with DMSO or the SB-202190 (A+C, 1–10 μM) and SB-203580 (D+E, 5–20 μM) 45 min prior to TGFβ addition. Fold activations relative to untreated samples were calculated.

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Molecular Cancer

ISSN: 1476-4598