Northern confirmation of KDR, α
integrin subunit, VE-cadherin, MMP1, and MMP2 during activation of ECs. ECs were cultured in 3D collagen matrices with VEGF165. At various time points (indicated), total RNA was isolated and analyzed by Northern blot, as described in the "Methods". Blots were hybridized with indicated 32P-radiolabeled probes. PCR primers used to generate probes are shown in Table II. Data shown are representative of those obtained in two or three separate experiments, with similar results.