Confirmation of gene expression as determined by Western blot analysis. EC monolayers were either left untreated (lane 1) or were treated with 100 ng/ml of bFGF (lane 2), VEGF (lane 3), or 20 ng/ml PMA (lane 4) for 48 hours. Total cellular protein was extracted and analyzed by Western blot, as described in the "Methods". Blots were incubated with: (A) anti-KDR; (B) anti-F1t-1; (C) anti-α2 integrin subunit; (D) anti-VE-cadherin; (E) anti-MMPl; (F) anti-MMP2; (G) anti-Kinesin; (H) anti-VCIP; and (I) anti-Grb2 antibodies. The molecular mass of each protein detected is indicated (kiloDaltons, kDa). Blots are representative of those obtained in two or three separate experiments, with similar results.