Figure 4From: Effects of STI571 (gleevec) on pancreatic cancer cell growthEffect of STI571 on growth factor-induced MAPK phosphorylation. Mia-PaCa-2 and T3M4 cells were cultured in 1% FCS medium overnight, then treated with 10 ng/ml of the indicated growth factors and GI50 concentrations of STI571 for 5 minutes. Phosphorylation of MAPK was determined by Western blot analysis with antibodies specific for phospho-p44/42 MAPK. Equal loading was determined by stripping the membranes and blotting with an ERK2 (p42) antibody. The figure is representative of three independent experiments.Back to article page