Figure 3
Paxillin and c-Jun are associated with focal adhesions. BxPC-3 cells were stimulated with 10 nM of mature TGFβ1 for ten minutes followed by preparation of the Triton-X100 nonsoluble fraction and precipitation with αVβ6 integrin antibodies. The precipitate was then re-precipitated with anti-FAK antibodies (sc-1688) and analyzed with antibodies against p-PaxillinY118 (2541, Cell Signaling) and p-c-JunS63 (9621, Cell Signaling). In part, the cells were preincubated with a TGFβ-RII antibody (Santa Cruz), BAPTA-AM and Cytochalasin D, respectively [10].