Figure 5

Anx-II mRNA expression, but not protein, is induced by 5-aza-2'-deoxycytidine, A: LNCaP and DU-145 cells were treated with or without 3 μM 5-aza-2'-deoxycytidine for 48 hours, and the annexin II expression was measured by RT-PCR. M: marker; lane 1: untreated LNCaP; lane 2: Aza-dC treated LNCaP; lane 3 untreated DU-145; lane 4 Aza-dC treated DU-145. B: LNCaP cells were treated with or without 3 μM 5-aza-2'-deoxycytidine for 48 hours. 10 μg of total protein was subjected to Western blot as described in Materials and Methods. 10 μg of whole cell extract of K562 cells was used as a positive control. Lane 1: treated LNCaP; Lane 2: untreated LNCaP; Lane 3: mock-treated LNCaP (with Lipofectamine); Lane 4: K562 cell extract. The bottom panel shows expression of an internal control in the same samples. PGK was used as an internal control.