Phenotype of human DC transduced with AdCCL-21. DC, Ad-CCl-21-DC and AdCV-DC were characterized by flow cytometry. On day 8, cells were stained with antibodies to cell surface markers conjugated to fluorochromes listed in Materials and Methods and FACS analysis was performed. Histograms show surface expression in non-transduced DC and AdCCl-21-DC transduced at MOI 50:1 and 100:1. Control DC in the first panels; Ad-CCL-21-DC at MOI in the second column; Ad-CCL21-DC at MOI of 100:1 in the third column; and AdCV-DC in the final panels. Dotted lines represent isotype control and bold lines the surface marker staining. Results are representative of three independent experiments.