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Figure 4 | Molecular Cancer

Figure 4

From: Overexpression of CCL-21/Secondary Lymphoid Tissue Chemokine in Human Dendritic Cells Augments Chemotactic Activities for Lymphocytes and Antigen Presenting Cells

Figure 4

Chemotactic activity of AdCCL-21-DC is blocked by anti-CCL-21 neutralizing antibody. T2 cells were used as APCs in transwell Chemicon assays. 2.0 × 105 T2 cells in 100 μl serum free medium were added to the upper chamber of the transwell plate. 150 μl of supernatant from AdCCL-21-DC were pre-incubated for 30 minutes with anti-CCL-21 antibody or with a control antibody prior loading to the lower chamber of the Chemicon apparatus. After 2 hours incubation at 37°C, cells were lysed and stained with a green fluorescent dye according to the manufacturer's instructions. Total cell migration was determined by measuring the fluorescence of cells collected from the lower chamber and the bottom of the inserts using a fluorescence plate reader with 480/520 nm filter set. Values are expressed as relative migration versus the control (10% AB medium) and are representative of three independent experiments.

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