Adenoviral vector expressing human CCL-21. The adenoviral construct (Ad-CCL-21) is an E1-deleted, replication-deficient adenoviral serotype 5 vector encoding a 491 bp human CCL-21 cDNA. The 491 bp-containing human CCL-21 coding sequence 59–453, was amplified by PCR with the following primers: 5' primer 5'-CTTGCAGCTGCCCACCTCAC-(1-20) and 3' primer 5'-TCTCCAGGGCTCCAGGCT-GC-3 (491-472). The CCL-21 gene was amplified and cloned into pAC-CMVpLpA (to generate pAC-CMV-CCL21pA) with confirmation using restriction analysis and sequencing. pAC-CMV-CCL21pA was co-transfected with pJM-17 (plasmid containing the E1-deleted Ad-genome) into 293 cells to yield recombinant E1-deleted adenovirus (AdCCL-21) following homologous recombination.