Skip to main content

Advertisement

Table 1 CCL-21 production by AdCCL-21-DC

From: Overexpression of CCL-21/Secondary Lymphoid Tissue Chemokine in Human Dendritic Cells Augments Chemotactic Activities for Lymphocytes and Antigen Presenting Cells

  DC AdCCL-21-DC MOI 50:1 AdCCL-21-DC MOI 100:1 AdCV-DC MOI 100:1
   ng/106 DC/48 hours   
Standard Method 0.5 ± 0.24 *71 ± 15 *91.2 ± 5 0.5 ± 0.02
Centrifugation Method 0.67 ± 0.004 *210 ± 8.9 *278.2 ± 16.5 0.78 ± 0.05
    *p ≤ 0.01; **p ≤ 0.0009  
  1. CCL-21 protein production by AdCCL-21-DC. DC propagated in vitro with GM-CSF and IL-4 were transduced with human AdCCL-21 or AdCV on day 6 of culture at MOIs 50:1 and 100:1. Transduction was accomplished by the standard incubation at 37°C (a) or by the centrifugation method (b) as described in materials and methods. CCL-21 protein concentration was determined in the supernatant of AdCCL-21 DC, AdCV-DC and NTDC by ELISA 48 hours after transduction. A 2.9 fold increase in CCL-21 protein production was achieved by the centrifugation method of DC transduction compared to the standard method. Values refer to CCL-21 protein concentration expressed in nanograms per million cells and are representative of three independent experiments. P values refer to significant differences of AdCCL-21-DC with AdCV-DC and control DC.