Figure 11

The SB503280 p38 inhibitor reduces p8 expression in Panc-1 and BxPc-3 cells. Panc-1 and BxPc-3 cells were treated with 10 μM of SB203580 for 12 hours and total RNA and protein were prepared and p8 mRNA and p8 protein expression were measured by RT-PCR and Western blot respectively as described in Material and Methods. RL3 mRNA expression was used as control in RT-PCR experiments and β-tubulin was used as a housekeeping control protein in Western blots.