p8 transcription is activated by TGFβ-1 through Smad4 in Panc-1 cells. Panc-1 cells were cultivated in 30 mm diameter culture dishes for 24 hours and then transiently transfected with 0.5 μg of p8-CAT and 0.5 μg of pCMV/βgal plasmids. Plamids encoding constitutively activated type I TGFβ receptor (RI ACT), dominant negative type II TGFβ receptor (RII DN), dominant negative of Smad4 (DPC4 1–514 a.a.) and the Smad4 wild type (0.5 μg) were co-transfected with p8-CAT and pCMV/βgal plasmids in the presence or not of TGFβ-1 (5 ng/ml) or the p38 inhibitor SB203580 as indicated. Cell extracts were prepared 24 hours after transfection and CAT and β-galactosidase activities were measured. CAT activity was normalized to β-galactosidase activity. Experiments were carried out in triplicate and repeated two or three times.