Figure 4From: p8 inhibits the growth of human pancreatic cancer cells and its expression is induced through pathways involved in growth inhibition and repressed by factors promoting cell growthCell growth arrest by serum deprivation induces p8 expression in Panc-1 and BxPc-3 cells. Panc-1 and BxPc-3 cells were cultivated in 10-cm Petri plates for 24 hours in a culture medium containing 0.1% fetal calf serum. Then, the culture medium was replaced by culture medium with 10% fetal calf serum to start cell growth or by fresh culture medium with 0.1% fetal calf serum to maintain arrest. Twenty four hours later cells were recovered and RNA and proteins prepared and p8 mRNA and p8 protein expression were estimated by RT-PCR and Western blot respectively as described in Material and Methods. RL3 mRNA expression was used as control in RT-PCR experiments and β-tubulin as a housekeeping control protein in Western blots.Back to article page