Cell growth arrest by serum deprivation induces p8 expression in Panc-1 and BxPc-3 cells. Panc-1 and BxPc-3 cells were cultivated in 10-cm Petri plates for 24 hours in a culture medium containing 0.1% fetal calf serum. Then, the culture medium was replaced by culture medium with 10% fetal calf serum to start cell growth or by fresh culture medium with 0.1% fetal calf serum to maintain arrest. Twenty four hours later cells were recovered and RNA and proteins prepared and p8 mRNA and p8 protein expression were estimated by RT-PCR and Western blot respectively as described in Material and Methods. RL3 mRNA expression was used as control in RT-PCR experiments and β-tubulin as a housekeeping control protein in Western blots.