Figure 5

p8 expression is regulated by the rasV12 mutated oncogene. BxPc-3 cells were transduced with pLPC-rasV12 and pLPC (control) retrovirus and selected with puromycin. Expression of p8 mRNA and p8 protein was estimated by RT-PCR and Western blot respectively as described in Material and Methods. RL3 mRNA expression was used as control in RT-PCR experiments and β-tubulin was used as a housekeeping protein control in Western blot experiments.