Figure 5From: p8 inhibits the growth of human pancreatic cancer cells and its expression is induced through pathways involved in growth inhibition and repressed by factors promoting cell growthp8 expression is regulated by the rasV12 mutated oncogene. BxPc-3 cells were transduced with pLPC-rasV12 and pLPC (control) retrovirus and selected with puromycin. Expression of p8 mRNA and p8 protein was estimated by RT-PCR and Western blot respectively as described in Material and Methods. RL3 mRNA expression was used as control in RT-PCR experiments and β-tubulin was used as a housekeeping protein control in Western blot experiments.Back to article page