Figure 8From: p8 inhibits the growth of human pancreatic cancer cells and its expression is induced through pathways involved in growth inhibition and repressed by factors promoting cell growthp8 transcription is repressed through the JNK pathway. Panc-1 and BxPc-3 were cultivated in 30 mm diameter culture dishes for 24 hours and then transiently transfected with 0.5 μg of p8-CAT and 0.5 μg of pCMV/βgal plasmids. Expression plasmid pcDNAIIIB HA JNK encoding the wild-type JNK (0.5 μg) was co-transfected with p8-CAT and pCMV/βgal plasmids as indicated. Cell extracts were prepared 24 hours after transfection and CAT and β-galactosidase activities were estimated. CAT activity was normalized to β-galactosidase activity. Experiments were carried out in triplicate and repeated two times.Back to article page