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Figure 8 | Molecular Cancer

Figure 8

From: p8 inhibits the growth of human pancreatic cancer cells and its expression is induced through pathways involved in growth inhibition and repressed by factors promoting cell growth

Figure 8

p8 transcription is repressed through the JNK pathway. Panc-1 and BxPc-3 were cultivated in 30 mm diameter culture dishes for 24 hours and then transiently transfected with 0.5 μg of p8-CAT and 0.5 μg of pCMV/βgal plasmids. Expression plasmid pcDNAIIIB HA JNK encoding the wild-type JNK (0.5 μg) was co-transfected with p8-CAT and pCMV/βgal plasmids as indicated. Cell extracts were prepared 24 hours after transfection and CAT and β-galactosidase activities were estimated. CAT activity was normalized to β-galactosidase activity. Experiments were carried out in triplicate and repeated two times.

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Molecular Cancer

ISSN: 1476-4598