Figure 9From: p8 inhibits the growth of human pancreatic cancer cells and its expression is induced through pathways involved in growth inhibition and repressed by factors promoting cell growthThe SP600125 JNK inhibitor increases p8 expression in Panc-1 and BxPc-3 cells. Panc-1 and BxPc-3 cells were treated with 10 μM of SP600125 for 12 hours and total RNA and protein were prepared and p8 mRNA and p8 protein expression were estimated by RT-PCR and Western blot respectively as described in Material and Methods. RL3 mRNA expression was used as control in RT-PCR experiments and β-tubulin was used as a housekeeping control protein in Western blots.Back to article page