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Figure 1 | Molecular Cancer

Figure 1

From: Analysis of CUL-5 expression in breast epithelial cells, breast cancer cell lines, normal tissues and tumor tissues

Figure 1

RT-PCR analysis for CUL-5 in HMEC, MCF-10A, MCF-7, and MDA-MB-231 cells. Semiquantitative RT-PCR was performed using 1 μg of total RNA with 20, 25, and 29 cycles of PCR using primers for (A) CUL-5, or (B) ribosomal protein S9; (C) The CUL-5 PCR products from HMEC, MCF-10A, MCF-7, and MDA-MB-231 cells were confirmed by restriction endonuclease digestion with EcoR I. The results shown are representative of triplicate experiments using independent RNA samples.

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