Figure 1

RT-PCR analysis for CUL-5 in HMEC, MCF-10A, MCF-7, and MDA-MB-231 cells. Semiquantitative RT-PCR was performed using 1 μg of total RNA with 20, 25, and 29 cycles of PCR using primers for (A) CUL-5, or (B) ribosomal protein S9; (C) The CUL-5 PCR products from HMEC, MCF-10A, MCF-7, and MDA-MB-231 cells were confirmed by restriction endonuclease digestion with EcoR I. The results shown are representative of triplicate experiments using independent RNA samples.