Northern blot analysis for CUL-5 in HMEC, MCF-10A, MCF-7, and MDA-MB-231 cells. Approximately 10 μg of total RNA from HMEC, MCF-10A, MCF-7, MDA-MB-231 was electrophoresed and transferred to a Nytran® supercharge membrane. The blots were hybridized with a 32P-labeled 674-bp CUL-5 cDNA or a 905-bp GAPDH cDNA. The results shown are from a 4-day exposure to x-ray film for CUL-5 and a 5-hr exposure to x-ray film for GAPDH. A major CUL-5 transcript of ~7.4 kb was present in all of the cells examined. The results shown are representative of triplicate experiments using independent RNA samples.