Expression of CUL-5 in matched normal tissue versus tumor tissue. The data in this figure was derived by quantification of the data in Figure 6 using a PhosphorImager and ImageQuant software. The mean volumes ± the SEM are shown. An asterisk indicates a statistically significant difference between the matched normal versus tumor tissue as determined by two-way paired t-tests (P ≤ 0.05). A statistically significant decrease in CUL-5 expression was found in breast tumor tissue (~2.2 fold decrease, P < 0.0001, n = 50), uterine tumor tissue (~1.5 fold decrease, P = 0.0021, n = 42), colon tumor tissue (~1.4 fold decrease, P = 0.0012, n = 35), and kidney tumor tissue (~1.5 fold decrease, P = 0.0034, n = 20), compared to matched normal tissues. A significant increase in CUL-5 expression was found in prostate tumor tissue compared to matched normal tissue (~1.5 fold increase, P = 0.0335, n = 4).