Skip to main content

Table 1 Primers used for RT-PCR analysis of CUL family members

From: Analysis of CUL-5 expression in breast epithelial cells, breast cancer cell lines, normal tissues and tumor tissues

Primer Sequences Primer Location
and GenBank Accession #
Annealing
Temp. and
Predicted
product size
Restriction
endonuclease
and cut site
CUL-1 forward:
5'-CGCTGGCTTTGTGGCTGCTC-3'
CUL-1 reverse:
5'-TGTGGCGGCTGGCGTAGAA-3'
nt 1510 – 1529
nt 2051 – 2069
XM027270
58°C
560-bp
Bgl II
1891
CUL-2 forward:
5'-CAGCGAAAGGGATGACAGAGAATG-3'
CUL-2 reverse:
5'-TGAGTAAGAGGCCACGCACCA-3'
nt 1351 – 1374
nt 1688 – 1708
XM005814.3
54°C
358-bp
Aat II
1435
CUL-3 forward:
5'-AGAGCGGAAAGGAGAAGTCGTAGA-3'
CUL-3 reverse:
5'-CTCAAAGTCACCCGCAATAGTT-3'
nt 833 – 856
nt 1424 – 1445
XM051985.1
53°C
613-bp
EcoR I
1160
CUL-4A forward:
5'-AACTCCACGCTGCCCTCCATCTG-3'
CUL-4A reverse:
5'-TGCTGCCCGCCCCTCACC-3'
nt 346 – 368
nt 840 – 857
XM027482.1
58°C
512-bp
Sau3A I
452
CUL-4B forward:
5'-GCGGAGCTGCTTTCACCA-3'
CUL-4B reverse:
5'-TTCTTCAACCGTTTCTTTCATCTG-3'
nt 1400 – 1417
nt 1969 – 1992
NM003588.1
54°C
593-bp
Hae III
1551
CUL-5 forward:
5'-GAGTGGCTAAGAGAAGTTGGTATG-3'
CUL-5 reverse:
5'-TCTTCTCTCATCCTTTCTGTAGTG-3'
nt 1521 – 1544
nt 2171 – 2194
NM003478.1
58°C
674-bp
EcoR I
1767
Ribosomal S9 forward:
5'-GCAGGCGCAGACGGGGAAGC-3'
Ribosomal S9 reverse:
5'-CGCGAGCGTGGTGGATGGAC-3'
nt 6 – 25
nt 397 – 416
NM001013.2
62°C
411-bp
Dde I
349