Analysis of CUL-5 expression in breast epithelial cells, breast cancer cell lines, normal tissues and tumor tissues

Fay, Michael J; Longo, Kenneth A; Karathanasis, George A; Shope, David M; Mandernach, Craig J; Leong, Jason R; Hicks, Alfred; Pherson, Kenneth; Husain, Amyna

doi:10.1186/1476-4598-2-40

Molecular Cancer

Table 2 Analysis of CUL-5 expression in normal human tissues and cancer cell lines using a multiple human tissue expression array. The array was hybridized with the ³²P-labeled (2 × 10⁷ cpm) 674-bp CUL-5 cDNA for 20 hrs. Analysis of the array was performed using a PhosphorImager and ImageQuant software. The background-adjusted volumes for each sample are reported for the 2-day exposure of the array to a phosphor-screen.

From: Analysis of CUL-5 expression in breast epithelial cells, breast cancer cell lines, normal tissues and tumor tissues

	1	2	3	4	5	6	7	8	9	10	11	12
A	whole brain 77451	cerebellum left 67297	substantia nigra 41295	heart 97252	esophagus 44513	colon, transverse 62300	kidney 137204	lung 58777	liver 85484	leukemia, HL-60 48972	fetal brain 43774	yeast total RNA
B	cerebral cortex 73105	cerebellum right 110567	accumbens nucleus 82337	aorta 31370	stomach 63062	colon, descending 60792	skeletal muscle 163430	placenta 80932	pancreas 79718	HeLa S3 32213	fetal heart 55979	yeast tRNA
C	frontal lobe 68310	corpus callosum 63455	thalamus 76840	atrium, left 83350	duodenum 54191	rectum 49102	spleen 53685	bladder 61953	adrenal gland 70467	leukemia, K-562 58024	fetal kidney 63966	E. coli rRNA
D	parietal lobe 63575	amygdala 72591	pituitary gland 65881	atrium, right 71626	jejunum 75472		thymus 50578	uterus 46700	thyroid gland 62460	leukemia, MOLT-4 40827	fetal liver 47640	E. coli DNA
E	occipital lobe 78745	caudate nucleus 84064	spinal cord 28621	ventricle, left 76376	ileum 66144		peripheral blood leukocyte 29858	prostate 57159	salivary gland 73592	Burkitt's lymphoma, Raji 31147	fetal spleen 62971	poly r(A)
F	temporal lobe 55023	hippocampus 52220		ventricle, right 62321	ileocecum 45452		lymph node 48852	testis 48162	mammary gland 28970	Burkitt's lymphoma, Daudi 30413	fetal thymus 53776	human C₀t-1 DNA
G	paracentral gyrus of cerebral cortex 48569	medulla oblongata 60577		inter-ventricular septum 89675	appendix 56027		bone morrow 37152	ovary 11139		colorectal adenocarcinoma, SW480 54931	fetal lung 64933	human DNA 100 ng 6117
H	pons 55401	putamen 70937		apex of the heart 91122	colon, ascending 40405		trachea 38285			lung, carcinoma, A549 27590		human DNA 500 ng 9541

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ISSN: 1476-4598

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