Table 2 Examples of some of the regulatory pathways affected by 5-FU exposure, and associated genes that were scored as up- or down-regulated in one or more HCT116 treatment-derivatives.
Regulatory pathway affected | Altered gene in one or more derivatives | Bolus3 signal log2 ratio | ContinB signal log2 ratio | ContinD signal log2 ratio |
|---|---|---|---|---|
5-FU-metabolism | DHFR | 0.6 | 0.7 | 1.0 |
| Â | DPYD | -1.0 | -1.5 | -0.9 |
| Â | DTYMK | 1.0 | 1.4 | 0.7 |
| Â | TYMS | 0.7 | 0.9 | 0.7 |
| Â | UP | 1.2 | 1.6 | (0.3) |
Apoptosis | ANXA4 | -1.0 | -1.2 | (-0.3) |
| Â | BIRC5 | 0.6 | 1.2 | 0.7 |
| Â | BNIP3L | -2.4 | -2.7 | -0.5 |
| Â | IRAK1 | 1.4 | 1.6 | 0.6 |
| Â | MALT1 | 0.6 | 1.5 | 0.8 |
| Â | PDCD4 | -2.8 | -2.9 | (0.0) |
DNA repair | FANCG | 2.1 | 2.3 | 1.7 |
| Â | FEN1 | 1.5 | 1.7 | 1.1 |
| Â | RAD23B | 2.3 | 2.3 | (0.2) |