Comparison of gene expression in HCT116 treatment derivatives generated by two different 5-fluorouracil exposure protocols

Table 2 Examples of some of the regulatory pathways affected by 5-FU exposure, and associated genes that were scored as up- or down-regulated in one or more HCT116 treatment-derivatives.

Regulatory pathway affected	Altered gene in one or more derivatives	Bolus3 signal log₂ ratio	ContinB signal log₂ ratio	ContinD signal log₂ ratio
5-FU-metabolism	DHFR	0.6	0.7	1.0
	DPYD	-1.0	-1.5	-0.9
	DTYMK	1.0	1.4	0.7
	TYMS	0.7	0.9	0.7
	UP	1.2	1.6	(0.3)
Apoptosis	ANXA4	-1.0	-1.2	(-0.3)
	BIRC5	0.6	1.2	0.7
	BNIP3L	-2.4	-2.7	-0.5
	IRAK1	1.4	1.6	0.6
	MALT1	0.6	1.5	0.8
	PDCD4	-2.8	-2.9	(0.0)
DNA repair	FANCG	2.1	2.3	1.7
	FEN1	1.5	1.7	1.1
	RAD23B	2.3	2.3	(0.2)

Signal log₂ ratios ≥ 0.5 defined an up-regulated gene, and signal log₂ ratios ≤ -0.5 defined a down-regulated gene. Values in parentheses represent 'no change' scores and are included here for comparison.

ISSN: 1476-4598