The promoter of the CHK2 gene is methylated in non-small cell lung cancers: A: The CHK2 gene promoter sequence from -615/-411 from the Genbank (AL117330). The putative transcriptional factor binding sites were underlined and potential CpG islands were bold and highlighted. B: Methylation specific PCR analyses of the genomic DNA isolated from the cancer cell lines: W- the wild type primers, M- methylation specific primers, U-unmethylation specific primers. The expected PCR product was 205 bps, and confimed by DNA sequencing analyses. C: Methylation specific PCR analyses of the genomic DNA isolated from the primary non-small cell lung cancer tumor specimens. Pt 7 was the normal match lung control from the patient 6 (tumor).