Figure 5
HIF-1α protein expression. Western blot analysis of HIF-1α protein expression in hypoxia-tolerant (M006x) or hypoxia-sensitive (M010b) cells. Lanes 1,4, cells incubated (24 h) then lysed under ambient aerobic conditions (18% O2); Lanes 2,5, cells incubated in hypoxia (0.6% O2 × 24 h) followed by 1 h re-oxygenation prior to lysis; Lanes 3,6, cells incubated in hypoxia (0.6% O2 × 24 h) then lysed in an atmosphere of 1% O2.