Table 1 mtDNA Sequence Analysis of Human GBM Cells. The mtDNA of hypoxia-tolerant (M006x, M006xLo, M059K) and hypoxia-sensitive (M010b) GBM cells was sequenced. Sequences that differ from the Cambridge sequence were checked against databases of known sequence polymorphisms available on the MITOMAP website [23]. A previously unreported mutation (T14634C) in the ND6 subunit of M010b cells was discovered. (insert C = a single "C" inserted into the D-loop poly-C tract; n.c. = no amino acid change; poly = polymorphism).

64

C-T

C-T

D-loop

transition

n.c.

Yes

152

T-C

D-loop

transition

H strand replication origin

Yes

195

T-C

D-loop

transition

n.c.

Yes

263

A-G

A-G

A-G

A-G

D-loop

transition

n.c.

Yes

303–309

insert C

insert C

D-loop

insertion

n.c.

Yes

311–315

insert C

insert C

insert C

D-loop

insertion

n.c.

Yes

320

C – T

D-loop

transition

replication primer (n.c.)

No

343

C – G

C – G

D-loop

transversion

n.c.

No

750

A – G

A – G

12S rRNA

transition

12S rRNA

Yes

4769

A-G

A-G

A-G

ND2

transition

met-met

Yes

8860

A-G

A-G

A-G

A-G

ATPase6

transition

thr-ala

Yes

8919

A-G

ATPase6

transition

glu-glu

No

11719

G-A

ND4

transition

gly-gly

Yes

14470

T-C

ND6

transition

gly-gly

Yes

14634

T-C

ND6

transition

met-val

No

14798

T-C

Cyt. B

transition

phe-leu

Yes

15326

A-G

A-G

A-G

A-G

Cyt. B

transition

thr-ala

Yes

16129

G-A

D-loop

transition

7S rRNA (n.c.)

Yes

16189

T-C

T-C

D-loop

transition

n.c.

Yes

16220

A-C

D-loop

transition

n.c.

Yes

16264

C-T

D-loop

transition

n.c.

Yes

16316

A-G

D-loop

transition

n.c.

Yes

16519

T-C

T-C

T-C

T-C

D-loop

transition

n.c.

Yes