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Table 4 Summary of RT-PCR details and the numbers of samples in each analysis. Analysis of hMLH1, IL-15 and Caspase2 restricted to smaller numbers due to limited availability of high-quality RNA.

From: Colorectal cancers with microsatellite instability display mRNA expression signatures characteristic of increased immunogenicity

Gene name

GENBANK NUMBER

Chemistry (Applied Biosystems, Warrington, UK)

Sample Numbers (n)

   

MSS

MSI-H

hMLH1

NM_000249

High-Capacity cDNA Archive Kit + TaqMan Universal PCR Master Mix (with AmpErase UNG)

12

16

TP53

NM_000546

TaqMan® EZ-RT-PCR Kit

26

27

HSP-70 kD 1B

NM_005346

TaqMan® EZ-RT-PCR Kit

26

28

HSP-110

NM_014278

TaqMan® EZ-RT-PCR Kit

26

28

IL-18

NM_001562

TaqMan® EZ-RT-PCR Kit

24

28

IL-8

NM_000584

TaqMan® EZ-RT-PCR Kit

26

28

IL-15

NM_000585

High-Capacity cDNA Archive Kit + TaqMan Universal PCR Master Mix (with AmpErase UNG)

12

16

Granulysin

NM_006433

TaqMan® EZ-RT-PCR Kit

26

27

Caspase 2

NM_001224

TaqMan® EZ-RT-PCR Kit

21

18