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Figure 1 | Molecular Cancer

Figure 1

From: Aberrant CBFA2T3B gene promoter methylation in breast tumors

Figure 1

CBFA2T3 gene structure and promoter methylation patterns. (A) CBFA2T3 encodes two alternative transcripts, CBFA2T3A and CBFA2T3B. CBFA2T3A is encoded by exons 1A and 2–12. CBFA2T3B is encoded by exons 1B-12 splicing out exon 3. Relative exon sizes are shown. The exon 1A start site contains no CpG island. The black box marks the location of a high-density CpG island located five prime to the exon 1B start site. The black arrowheads mark the primers used for real-time RT-PCR. (B) CBFA2T3B contains a CpG island of approximately 160 CpG sites spanning 1-kb of sequence. The single black bars represent CpG sites scaled relative to each other. CAT ELISA promoter constructs and primers used for MSP, real-time MSP and bisulfite sequencing are shown. The asterisk marks the location of the amplicon and internal primers used for second-round real-time MSP. (C) CBFA2T3B promoter methylation patterns were examined in hypermethylated cell lines using sodium bisulfite sequencing. A characteristic sinusoidal pattern of approximately six high to low frequency methylation levels every 40–150 bp was detected. The high-frequency cytosine methylation levels residing within a consensus Sp1 binding site located approximately minus 450 bp from the transcriptional start of exon 1B are shown.

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