Skip to main content
Figure 6 | Molecular Cancer

Figure 6

From: N-Methyl-N'-nitro-N-nitrosoguanidine-induced senescence-like growth arrest in colon cancer cells is associated with loss of adenomatous polyposis coli protein, microtubule organization, and telomeric DNA

Figure 6

APC mRNA levels in HCT-116 cells treated with MNNG. HCT-116 cells were treated with different concentrations of MNNG (0–100 μM) either for 15 h or for 50 h. After the treatment, the total RNA was isolated for Northern blot analysis as described in Materials and Methods. Panel A shows a representative autoradiogram of APC mRNA and corresponding 18 S RNA after 15 h of MNNG (0–100 μM) treatment. Panel B shows a representative autoradiogram of APC mRNA and corresponding 18 S RNA after 50 h of MNNG (0–100 μM) treatment. The level of 18 S mRNA was used for normalizing loading errors and transfer efficiency.

Back to article page

Molecular Cancer

ISSN: 1476-4598